FastQCFastQC Report
Sun 21 May 2023
EGAF00007931369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512425
Sequences flagged as poor quality0
Sequence length44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC38130.7441088939844855No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA35230.6875152461335805No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT33650.6566814655803288No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA31180.6084792896521443No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26610.5192955066595112No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25090.4896326291652437No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT23970.46777577206420456No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22900.4468946675123189No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA22750.4439674098648583No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20930.4084500170756696No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20390.39791188954481144No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19240.3754695809142802No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18320.3575157340098551No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG17130.334292823340001No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG17040.3325364687515246No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15880.3098990096111626No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14900.29077425964775333No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA13260.25876957603551737No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13070.255061716348734No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12960.25291506074059616No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11780.22988730058057277No Hit
TACCAGCTCCTGGCCCGGTTTGATCATGTTTTTATCCGCCAGTT11480.22403278528565157No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG11310.22071522661852955No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA10320.20139532614528954No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10280.20061472410596673No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10220.1994438210469825No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9800.1912474996340928No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9790.19105234912426208No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9360.18266087720154167No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT9280.18109967312289602No Hit
CAGCACGACTTTAGACGCTTTGTTACCGTATTTAACAACATAGG9210.17973361955408107No Hit
TTTAACAATCGCTTTAACCGCTTCTTCACCTTCCACCGCAACAA9150.17856271649509683No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9020.17602575986729765No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8610.16802458896423866No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8510.16607308386593161No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT8420.16431672927745522No Hit
GAACGCGTTCTGCTGCTCCTTGTTAAATTTGTTATCAGCTTTTG8260.16119432112016394No Hit
ACGCACGATTGCTTTGACTGCCTCTTCACCTTCAACGGCAACCA8130.15865736449236476No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC8070.1574864614333805No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT7340.14324047421573888No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT7280.14206957115675464No Hit
CCAGAAGGTACGGATCTGGTTTTTGCGTTTATCGTTCAGGGCAC7090.13836171146997123No Hit
TTCATAGAATGCGTTCTGCTGCTCTTTGTTGAACTTGTTATCCG6980.13621505586183344No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6780.13231204566521929No Hit
TTTGACAATTGCTTTGACAGCCTCTTCACCTTCCACAGCGACTA6500.1268478313899595No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6230.12157876762453042No Hit
GAATTTGTTTTGCTGAGCGTCGGCCTTCGGTGCTTGGGAATCAT6230.12157876762453042No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC6200.1209933160950383No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6130.11962726252622334No Hit
ACGATCGCGGCCCTGGAAGTTAACTTTCCACTGAGTCGGGATTT6040.11787090793774699No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5960.11630970385910133No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5870.11455334927062497No Hit
TTTCACGATAGCCTTTACCGCTTCTTCGCCTTCAACAGCGACAA5740.11201639264282579No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5670.11065033907401083No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5600.10928428550519588No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT5510.10752793091671953No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT5440.10616187734790457No Hit
TTTGGCTTCACCCAGGACGTTGGTGGACTGAGACGGATCGTCCT5370.10479582377908962No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5190.10128311460213689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)