FastQCFastQC Report
Sun 21 May 2023
EGAF00007931370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512425
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57911.1301166024296239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43390.8467580621554375No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA42050.8206078938381227No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA36470.7117139093525882No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34920.6814655803288285No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA32950.6430209298921794No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26550.5181246036005269No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA25560.498804703127287No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25020.4882665755964287No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC24210.4724593843001415No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG23850.465433965946236No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23000.4488461726106259No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21360.41684148899839No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT21070.41118212421329947No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA20850.406888812997024No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18360.35829633604917793No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG18070.35263697126408744No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16760.32707225447626487No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16600.3239498463189735No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG13630.26599014489925354No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13530.2640386398009465No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13010.2538908132897497No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG12480.24354783626872223No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG11350.2214958286578524No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11200.21856857101039176No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11170.21798311948089963No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG10350.20198077767478168No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG10250.2000292725764746No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10100.19710201492901402No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9540.1861735863784944No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT9110.17778211445577402No Hit
GTTAAAGTTATCGCGACTGAGGGTGGCGTAC9060.17680636190662047No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8940.174464555788652No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT8800.1717324486510221No Hit
AACGGTTTTGTAGCTTGGGATTCCCAGTACA8680.1693906425330536No Hit
GAACAGCAGAATGCGTTCTACGAAATTCTGC8110.15826706347270333No Hit
GTTACCATTATCGCGGGTGAAGGTAACGTCA8020.15651070888422694No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7980.15573010684490413No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA7650.14929014002049082No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7370.143825925745231No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7350.1434356247255696No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC7170.13992291554861688No Hit
CAGAACGCATTCTACGAAATCCTGCACCTGC7140.13933746401912475No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7050.13758110943064839No Hit
ACCGACGTTCTGGAATCCGCGACTGCAGACA6840.13348294872420355No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6620.129189637507928No Hit
GTTAAGGTGGTAAGCGGTGAAGGTAACGTTA6450.12587207884080598No Hit
ATGGAAACCCTGCTGATCGACAACGATGGCT6160.12021271405571547No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6140.11982241303605407No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5800.11318729570181003No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5630.109869737034688No Hit
GTAACCGTCATTGCGCAGCAGGGTGCAGTTC5550.10830853295604236No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA5550.10830853295604236No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG5510.10752793091671953No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC5510.10752793091671953No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5430.10596672683807387No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5420.10577157632824315No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5320.1038200712299361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)