FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528781
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT85601.6188176201489841No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43430.8213230051760559No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23960.45311764227534645No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG21630.4090540318203566No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20760.39260109572772095No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG20040.37898487275450515No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19130.3617754798300241No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18920.35780408146283627No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA16950.3205485824944542No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT16740.3165771841272663No Hit
CTGTCCTCCATGGCTATCACCTTCAAACGCG16700.315820727295421No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC15560.2942617075878293No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC14540.2749720583757737No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT12240.23147579054466783No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11840.22391122222621462No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11790.222965651186408No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11760.22239830856252402No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11360.21483374024407081No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11050.20897119979726955No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10720.2027304309345457No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10460.1978134615275511No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9950.18816863692152327No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT9720.1838190101384127No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9450.17871292652345677No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9310.17606532761199814No Hit
GTTCTGGTTAACGAAGGTAACGGTTATGTGG8500.16074707676713043No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8270.15639744998401983No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8080.1528042800327546No Hit
TCTGTGCGCGCAGCTGTATTCGCCACCGCGG8000.15129136636906393No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7780.14713085379391466No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7720.1459961685461467No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7630.14429414067449473No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7270.13748602918788685No Hit
CTGTCTTCCATGGCTATTACCTTCAAGCGTG7260.13729691497992552No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6840.12935411824554968No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6720.12708474775001372No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6660.12595006250224575No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC6590.12462626304651642No Hit
GTCGCGGGTAAACGTGAAGGTCTGGGCGGTC6520.12330246359078711No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6260.11838549418379254No Hit
CTGTCCTCTATGGCAATCACCTTCAAACGTG6190.11706169472806321No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6100.11535966685641125No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6090.11517055264844993No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6050.11441409581660461No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5890.11138826848922333No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA5870.11101004007330066No Hit
GAACTGGCTCGCCGTCAACAGGAAGAACGTA5840.11044269744941668No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT5810.10987535482553268No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA5800.10968624061757136No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5750.10874066957776471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)