FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541338
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA97801.8066346718685924No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA61281.132009945727069No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA56241.0389073000602211No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20720.3827553210748183No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA20170.37259531013895203No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19670.36335893656089174No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18310.3382360004285677No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17700.3269676246633342No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14900.27524393262619656No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13980.25824900524256567No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13770.25436972833978033No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13410.2477195393635769No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC12650.23368025152492528No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11920.22019514610095728No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11900.21982569115783485No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11310.2089267703357237No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11200.20689476814855046No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10790.199320941814541No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC10610.1959958473264393No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG10360.19137766053740915No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9460.17475218809690066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.17272018590972738No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT9060.16736308923445242No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8920.16477690463259553No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC8450.15609471346921885No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG8300.1533238013958008No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8280.15295434645267836No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8150.15055288932238267No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7950.14685833989115857No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG7920.14630415747647493No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7810.14427215528930168No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7690.14205542563056722No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7660.14150124321588362No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7590.14020815091495517No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7500.13854560367090432No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7470.13799142125622071No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7460.13780669378465948No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7400.13669832895529227No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7150.13208014216626213No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC7100.1311565048084561No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6830.12616886307630354No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6800.1256146806616199No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6710.12395213341756905No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6640.12265904111664061No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6480.11970340157166134No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6450.11914921915697771No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6340.11711721696980446No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6300.11637830708355963No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6290.11619357961199842No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG6220.11490048731106997No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA6200.11453103236794757No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5940.10972811810735622No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5660.10455574890364246No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5530.10215429177334678No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5470.10104592694397956No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5470.10104592694397956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)