FastQCFastQC Report
Sun 21 May 2023
EGAF00007931384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528568
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA94091.780092627627855No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA56571.070250185406608No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA55531.0505743821041No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42650.8068971258191945No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20580.3893538768900123No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA18880.3571915061070666No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18310.3464076523739613No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18010.3407319398828533No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17670.3342994657262642No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14310.270731485825854No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13560.2565422045980839No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13120.24821782627779207No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12800.24216373295394347No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC11580.21908250215677078No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11480.21719059799306806No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11280.21340678966566268No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11150.2109473142528492No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10870.20564998259448172No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC10320.19524450969411694No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10140.19183908219945212No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9610.1818119901318279No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9340.17670384888983065No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8480.16043347308198755No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC8020.1517307139289552No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7960.1505955714307336No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG7950.15040638101436332No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT7950.15040638101436332No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7720.14605500143784717No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7670.14510904935599583No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7460.14113605061222018No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7440.14075766977947965No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7420.1403792889467391No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7420.1403792889467391No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7390.13981171769762832No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7360.13924414644851751No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7320.13848738478303643No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7280.13773062311755535No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7260.13735224228481482No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7250.13716305186844455No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6710.12694676938445004No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6650.12581162688622846No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6420.12146024730971229No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6420.12146024730971229No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6390.12089267606060147No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6360.12032510481149065No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6260.11843320064778798No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6250.11824401023141771No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6200.11729805814956638No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG6030.11408182107127182No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6010.11370344023853127No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA5880.1112439648257178No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG5780.1093520606620151No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5430.10273039608905571No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5360.10140606317446384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)