FastQCFastQC Report
Wed 31 May 2023
EGAF00007931401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377465
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA50221.3304544792232393No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA46041.2197157352337304No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG28940.7666936007311936No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC27150.7192719854820976No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA21680.574357887486257No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18400.4874624137337236No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18330.48560793715973666No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17500.463619143496748No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA17420.4614997416979058No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13400.3549998013060814No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC13350.35367517518180497No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13000.34440279231187No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12360.32744757792113177No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG11470.30386923290901147No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT10830.2869140185182732No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT10500.2781714860980488No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10150.2688991032281139No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9840.26068642125760005No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9640.2553879167604944No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9520.25220881406223095No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9420.2495595618136781No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9280.2458506086657041No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8740.23154464652351872No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8510.2254513663518472No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8270.21909316095532036No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8030.21273495555879351No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7780.2061118249374114No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7230.19154093757037077No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6850.18147377902586995No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6510.17246632138079027No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6410.16981706913223743No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5740.15206707906693337No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5690.15074245294265692No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5690.15074245294265692No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5600.14835812591895936No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4930.1306081358536553No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4850.12848873405481304No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4750.12583948180626017No Hit
CAGGGTTTTCAGGAAAACCAGCGGCGGACGCTTCTGCAGACCTT4360.11550739803690409No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG4280.11338799623806181No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4010.10623501516696912No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG4010.10623501516696912No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4010.10623501516696912No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG3930.10411561336812684No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC3910.10358576291841627No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG3780.10014173499529758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)