FastQCFastQC Report
Sun 21 May 2023
EGAF00007931404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413722
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT133923.2369562169766173No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC41711.0081649030024993No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT31170.75340445999971No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27070.654304097920826No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24080.5820333460632985No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22120.5346585388255882No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19630.4744731969776807No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19130.46238778696806065No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16160.39060045151091793No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15600.3770647923001436No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15110.365221090490716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13900.3359743982674356No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG13340.32243873905666126No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12460.30116841743973005No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11810.28545738442722407No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC11440.2765141810201053No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11150.2695046432145257No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11110.2685378104137561No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10650.2574192332049057No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10490.2535519020018273No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10350.25016798719913375No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA10210.24678407239644012No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9870.23856599358989855No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8700.21028613416738778No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8670.20956100956681056No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8220.1986841405581526No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7820.18901581255045657No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7260.1754801533396822No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG7220.1745133205389126No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7200.1740299041385278No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6940.16774549093352542No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6680.16146107772852303No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG6480.15662691372467502No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5820.14067417251197664No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5660.13680684130889822No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA5480.13245609370543507No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5430.13124755270447305No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5070.12254605749754666No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4930.11916214269485306No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4930.11916214269485306No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4890.11819530989408346No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4860.11747018529350627No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4860.11747018529350627No Hit
ATGGCACTGATCAGCCAGTCCATTAAAAACC4380.10586819168427108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)