FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007931408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416732
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59561.4292158989470451No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23200.5567127074474723No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16100.38633942197863375No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13510.3241891671385927No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13500.3239492047646929No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13370.32082969390399585No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13000.31195108606970423No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12970.31123119894800494No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12760.30619198909610973No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11960.28699499918412796No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11230.2694777458894445No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC10890.2613190251768523No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8960.2150062870141962No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG8890.21332655039689777No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8420.20204831882360846No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8340.2001286198324103No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7730.18549091502452414No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7320.17565245769463347No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7160.17181305971223712No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6920.16605396273864256No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6860.16461418849524395No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG6570.15765527965215054No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6430.15429580641755375No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6280.15069637080905715No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6130.14709693520056055No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6010.1442173867137633No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5730.13749844024456964No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5520.13245923039267443No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5440.13053953140147626No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5340.1281399076624785No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5270.12646017104518012No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5160.12382058493228261No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5080.12190088594108443No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5010.12022114932378604No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4980.11950126220208672No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4940.11854141270648763No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT4920.11806148795868807No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4850.11638175134138966No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4730.1135022028545924No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4730.1135022028545924No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4720.11326224048069262No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4670.11206242861119377No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4600.11038269199389536No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4490.10774310588099786No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4450.10678325638539878No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG4400.10558344451589992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)