FastQCFastQC Report
Sun 21 May 2023
EGAF00007931410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428042
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44951.0501305946612716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21400.49995093939379776No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12980.30324127071642504No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10130.23665901944201737No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9750.22778138593876301No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9680.22614603239868983No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9510.222174459515655No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9140.21353044794669682No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8880.20745627765499647No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8120.18970101064848777No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7530.1759173165250139No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7320.1710112559047944No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7250.16937590236472122No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6920.16166637853294769No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6880.16073189079576303No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6740.15746118371561668No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6500.15185425729250868No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5960.13923867284051566No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5950.13900505090621948No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5910.13807056316903482No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5880.13736969736614632No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5650.13199639287733447No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5620.13129552707444597No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5550.1296601735343728No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5460.12755757612570728No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5440.12709033225711497No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC5370.12545497871704178No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5300.12381962517696861No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5240.12241789357119162No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5030.11751183295097212No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4900.11447474780512193No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4770.11143766265927177No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG4640.10840057751342158No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4630.10816695557912541No Hit
CAGGCGCCGTACCCGTCTGAGGACTCCCCGA4570.10676522397334841No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4530.10583073623616374No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4500.10512987043327525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)