FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007931412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436451
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46651.0688485076217031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14660.3358910851389961No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13920.3189361463257044No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9920.22728782841601922No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9770.223851016494406No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9760.2236218956996318No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9670.2215598085466639No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9620.22041420457279282No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9330.21376970152434063No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7790.17848509912911187No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7710.17665213277091815No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7700.17642301197614393No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7680.17596477038659553No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7160.16405048905833644No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT7150.16382136826356225No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6810.156031261241239No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6750.15465653647259373No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6320.14480434229730255No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6090.13953456401749564No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6060.13884720163317302No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6010.13770159765930196No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5980.13701423527497933No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5460.12509995394672024No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5460.12509995394672024No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5290.12120490043555864No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5120.11730984692439701No Hit
CAGGCGCCGTACCCGTCTGAGGACTCCCCGA5040.1154768805662033No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5010.11478951818188067No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4980.11410215579755803No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4950.1134147934132354No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4850.11112358546549327No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC4830.11066534387594484No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4730.10837413592820272No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4710.1079158943386543No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4690.10745765274910585No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG4630.10608292798046058No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4590.10516644480136374No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4590.10516644480136374No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4520.10356259923794424No Hit
CGTGACTCCGCATGGGACGTTCGTCCGCTGA4480.1026461160588474No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA4400.10081314970065368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)