FastQCFastQC Report
Sun 21 May 2023
EGAF00007931414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404732
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45121.1148117766818537No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30250.747408161450046No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG25300.6251050077582202No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22400.553452655090282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20950.5176264787563128No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20060.4956366187996007No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19730.48748307522014567No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19030.4701876797485744No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18810.46475198402893764No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18070.4464682802447052No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17590.43460858049277057No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12790.31601158297342435No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12050.29772787918919186No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11930.2947629542512082No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11500.28413863989010013No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8610.21273336430032713No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8230.20334443533004554No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7980.19716750837591293No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7560.18679027109297017No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7300.18036626706067224No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7010.1732010317938784No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6800.16801241315240703No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6640.16405917990176214No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6510.1608471778856132No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6400.15812933002579485No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6360.15714102171313363No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6170.15244655722799286No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6080.1502228635245051No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5870.1450342448830337No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5530.13663362422541336No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5490.13564531591275214No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5470.13515116175642153No Hit
CGTGGCGAACAGCAGTGGCTGGCAGGCAACC5280.13045669727128076No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT5150.1272446952551318No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5030.12427977031714815No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4970.1227973078481563No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4780.11810284336301552No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4050.1000662166569483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)