FastQCFastQC Report
Sun 21 May 2023
EGAF00007931416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376611
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59251.573241355138326No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT41701.1072432828568468No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29220.7758668759011288No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG23310.6189410293379641No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20430.5424695508097215No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18420.48909883142021876No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17920.4758225330646211No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17110.45431492972855286No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17110.45431492972855286No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16830.4468802026494181No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16550.43944547557028335No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11410.30296512847473916No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11330.3008409207378436No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11180.2968580312311642No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10910.2896888301191415No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8210.217996818998914No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7690.20418946870909238No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7590.20153420903797287No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7270.19303737809039037No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6660.17684029409656118No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6420.17046767088587428No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6270.16648478137919498No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6130.16276741783962762No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5860.15559821672760488No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5530.14683585981291042No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5440.1444461261089028No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5350.14205639240489523No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5310.1409942885364474No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5240.13913560676666376No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4890.12984219791774537No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4840.12851456808218562No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT4840.12851456808218562No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4550.12081431503593895No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4540.12054878906882698No Hit
CGTGGCGAACAGCAGTGGCTGGCAGGCAACC4360.11576932166081182No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4330.11497274375947596No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4120.10939669845012492No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG3940.10461723104210977No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG3830.10169644540387826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)