FastQCFastQC Report
Sun 21 May 2023
EGAF00007931427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403533
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21800.5402284323710824No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21050.5216425918078571No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19900.493144302944245No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG18790.4656372589106715No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG18070.4477948519699752No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG15740.3900548406202219No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15360.38063801473485437No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13620.3375188646281717No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12750.31595928957483027No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12330.3055512188594241No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG12160.3013384283317597No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG11320.2805222869009474No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10360.256732410980019No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG10300.25524554373496094No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10170.2520239980373352No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9570.237155325586755No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9480.23492502471916796No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7890.1955230427251303No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7300.18090218148205972No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7030.17421127887929858No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6890.17074192197416319No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA6730.16677694265400847No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6710.16628132023898912No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6530.16182071850381505No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA6400.15859917280618932No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6330.15686449435362163No Hit
CAGGATCATCAGGCAATCGGCCAGAGCGCGATGAGCACCCGCAG5940.14719985726074447No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5640.13976552103545434No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5510.13654397533782864No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5430.13456148567775125No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5320.13183556239514488No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5240.12985307273506752No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5080.12588809341491278No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4970.1231621701323064No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4530.1122584770018809No Hit
TGGGGTACCGGATACCTGTTTTTCCAGGCGACCCTGAACCGGAG4450.11027598734180352No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4410.10928474251176484No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4250.10531976319161011No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4250.10531976319161011No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4140.10259383990900371No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4080.10110697266394571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)