FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441911
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58801.330584665238023No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21620.489238783374933No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21240.48063976683087767No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20650.46728866219668663No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG19900.4503169190176302No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16210.36681594257667266No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15370.3478075902161295No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14990.33920857367207424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14590.3301569773099108No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14030.31748474240288205No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG13860.31363781394896256No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT12710.28761447440774274No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12340.2792417477727416No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA11620.2629488743208474No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11150.2523132485953054No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11090.25095550914098086No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10820.24484568159652054No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10340.23398376596192447No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10250.2319471567804377No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9070.20524494751205558No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9040.20456607778489333No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8360.1891783639692155No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8160.1846525657881338No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7490.16949114188151007No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7310.16541792351853654No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6880.15568745742921086No Hit
ACCCGCCACATTGACGCAAAAGCAGTTTACG6870.15546116752015676No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6640.1502564996119128No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6600.14935133997569647No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6150.13916829406826262No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6090.1378105546139381No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6060.13713168488677585No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5660.12808008852461242No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5320.12038623161677352No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA5170.11699188298096223No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5090.11518156370852954No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4860.10997689580028558No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4860.10997689580028558No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4730.10703512698258247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)