FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412078
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42881.0405796960769562No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27870.6763282679492717No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24580.5964890142157553No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC24130.5855687515470372No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22680.5503812385033902No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22280.5406743383534186No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT18670.4530695644999248No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16530.4011376486975767No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16340.39652687112634016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13950.33852814273025983No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12270.29775916210037906No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12030.2919350220103961No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11680.28344148437917094No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11530.2798013968229316No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11460.2781026892966866No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11130.27009449667296No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10420.25286474890676036No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10380.2518940588917632No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10020.24315784875678878No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9850.23903241619305082No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9200.22325870344934695No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8520.20675697319439526No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8180.19850610806691937No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7830.1900125704356942No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7810.18952722542819564No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7360.17860696275947757No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7270.17642291022573398No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7190.17448153019573964No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7060.17132678764699888No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG6970.16914273511325525No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6780.16453195754201874No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6670.16186256000077656No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6580.15967850746703294No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6310.15312634986580212No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6130.14875824479831487No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6120.1485155722945656No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5910.14341944971583048No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5820.14123539718208689No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5790.140507379670839No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5750.13953668965584184No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5680.13783798212959683No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5500.1334698770621096No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5440.13201384203961386No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5390.1308004795208674No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5340.12958711700212097No Hit
ATGAGCCACCAGAGCCTGGACGATGACGCTG5110.12400564941588729No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4590.1113866792209242No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4590.1113866792209242No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4430.10750391916093555No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC4400.10677590164968767No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4360.10580521163469053No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4320.10483452161969337No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4210.10216512407845116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)