FastQCFastQC Report
Sun 21 May 2023
EGAF00007931450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375515
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62491.6641146159274596No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53921.435894704605675No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26260.6993062860338468No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24700.6577633383486678No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC19120.5091674100901429No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17710.4716189766054618No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16740.4457877847755749No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15480.4122338654913918No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14730.3922612944889019No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12430.33101207674793287No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12420.330745775801233No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11240.2993222640906488No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11000.2929310413698521No Hit
AACTCTCCAACCGCTGAAGAGTGCGGTTACT9850.2623064324993675No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9820.26150752965926793No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9740.259377122085669No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9440.251388093684673No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9320.24819248232427465No Hit
AACTCTCCAACTGTTGAAGAATGTGGTTATA8430.22449169806798663No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7470.19892680718479958No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG7470.19892680718479958No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7370.1962637977178009No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7070.18827476931680492No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6880.18321505132950747No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6730.17922053712900948No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG6480.17256301346151287No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6420.17096520778131366No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6050.16111207275341863No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5950.15844906328642No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5670.15099263677882377No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5590.14886222920522482No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5310.1414058026976286No Hit
AATTCCCCGAACATCGAAGCGTGCGGCTATA5240.13954169607072955No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4730.1259603477890364No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA4540.12090062980173895No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4490.1195691250682396No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4400.11717241654794082No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4330.11530830992104177No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG4020.10705298057334593No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4020.10705298057334593No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4010.10678667962664608No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG3790.10092805879924904No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC3770.1003954569058493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)