FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382102
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59431.5553438610632762No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA39581.0358490664796312No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22670.5932970777436392No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG21790.5702665780341375No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG20950.5482829192205223No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20510.5367676693657715No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18470.4833787836755631No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17300.4527586874708847No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17100.4475244829914526No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT16050.4200449094744335No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15860.4150724152189729No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15850.41481070499500133No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14420.37738614296706113No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14190.37136680781571413No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13780.3606366886328781No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12020.31457568921387485No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11730.30698609271869814No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT11530.30175188823926596No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10740.28107678054550883No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10330.27034666136267277No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC9780.2559525990442343No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC9590.25098010478877364No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9050.23684775269430677No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9010.2358009117984203No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8960.23449236067856227No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8860.23187525843884615No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8380.2193131676882089No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8080.2114618609690606No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8000.20936817917728773No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7600.19889977021842337No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7230.1892164919314738No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7150.18712281013970092No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6690.17508413983700685No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA6290.1646157308781425No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6170.16147520819048317No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG6090.15938152639871028No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5670.1483896969919027No Hit
GAAGATTCCGTCTTCACTTCCACCCGTGCGC5650.14786627654395948No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5470.1431554925124705No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5460.1428937822884989No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5360.1402766800487828No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5360.1402766800487828No Hit
GGCGGCGGTGGCGCAAGCGGTGGCGGCGGTG5060.1324253733296345No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA5040.1319019528816913No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5040.1319019528816913No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5020.13137853243374806No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4960.1298082710899184No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4860.12719116885020232No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4780.12509748705842944No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4740.124050646162543No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4740.124050646162543No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4730.12378893593857138No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4630.12117183369885529No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC4610.12064841325091205No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4600.12038670302694046No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4550.1190781519070824No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4540.11881644168311079No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG4350.11384394742765022No Hit
TCTTCCGCTACTGCATGCACCGCAGGTGTTA4350.11384394742765022No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4330.11332052697970699No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG4250.1112268451879341No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4200.10991829406807607No Hit
GGCGCACAGGAAGAGCGCGAAGATACTGTAA4200.10991829406807607No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4170.10913316339616125No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4170.10913316339616125No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA4140.1083480327242464No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4140.1083480327242464No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG3980.10416066914070066No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG3930.1028521180208426No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC3900.10206698734892777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)