FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418515
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG25520.6097750379317348No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT22910.5474116817796255No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG22570.5392877196755194No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21360.5103759721873767No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC19600.46832252129553303No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19540.46688888092422015No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19370.4628268998721671No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17230.41169372662867526No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16880.40333082446268353No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13730.3280647049687586No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13320.31826816243145406No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT12700.3034538785945545No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12530.29939189754250145No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG12430.2970024969236467No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG11930.2850554938293729No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11400.2723916705494427No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT10800.25805526683631413No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG10680.25518798609368837No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10370.24778084417523866No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9920.23702854139039223No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9770.23344444046211008No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9530.22770987897685865No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9320.22269213767726367No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9220.2203027370584089No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8740.20883361408790607No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8590.20524951315962392No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8190.19569191068420488No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG8170.1952140305604339No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8160.19497509049854844No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7900.18876264888952604No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7890.18852370882764058No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7830.18709006845632772No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7480.17872716629033608No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG7290.17418730511451203No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7070.16893062375303156No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6880.1643907625772075No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA6700.16008984146326893No Hit
TTCGGACGGTTTTTTGGCAGCACCGGTGCCACCGGTTTCACGAA6610.15793938090629966No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6540.15626680047310132No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6180.1476649582452242No Hit
GCGCAGTGGCGGGGAGCCGTCGTTTTCGGAGGACGGCGGCGGAC6160.14718707812145324No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6060.14479767750259848No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5890.14073569645054537No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5770.13786841570791966No Hit
GTAAGCCAGTACCGCGCGACGGTTTTTGGAGTATGCCGCTTCGT5680.1357179551509504No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5620.1342843147796375No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5570.13308961447021014No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5570.13308961447021014No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5330.12735505298495872No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5200.12424883218044754No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5110.12209837162347825No Hit
GATGTCACCCAGTTCGGTCAGCACATCTTTTTCCGGGTTTTCGT4970.11875321075708158No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT4930.11779745050953967No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4920.11755851044765421No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4810.11493016976691396No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4690.11206288902428825No Hit
GTCCAGGTGCATGGTGTCACGGATTTTCTGGTACACGGCAGAAG4690.11206288902428825No Hit
AGATTCCGCCTTCAGACGACCACGGGTCATAACGCCCGCCGTGC4680.11182394896240277No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC4630.11062924865297541No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4630.11062924865297541No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4570.10919560828166254No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4490.10728408778657872No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4470.10680620766280778No Hit
GTATGCCAGTACCGCACGACGGTTTTTGGAGTAGGCAGCTTCAT4420.10561150735338042No Hit
CTGCACGGTAGCTACCATCGGGACCAGGTTGCGGGCCAGAATGC4340.1036999868582966No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4310.10298316667264017No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4220.10083270611567088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)