FastQCFastQC Report
Sun 21 May 2023
EGAF00007931454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418515
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA44821.0709293573707035No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25770.6157485394788718No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG25110.5999784953944303No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24110.5760844892058827No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23990.573217208463257No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21060.5032077703308125No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19120.4568533983250302No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18310.4374992533123066No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18250.4360656129409938No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17860.4267469505274602No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17370.41503888749507184No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16500.3942511021110355No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15970.3815872788311052No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14290.34144534843434526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14140.3378612475060631No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13540.32352484379293456No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT13470.3218522633597362No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12560.3001087177281579No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12460.2977193171093031No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC11360.2714359103019008No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11360.2714359103019008No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC11310.27024120999247336No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10040.23989582213301797No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9940.23750642151416315No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9740.23272762027645366No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9420.22508153829611843No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9290.2219753174916072No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9110.21767439637766867No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8900.2126566550780737No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8230.19664767093174676No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8180.1954529706223194No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8020.19162992963215178No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7720.1844617277755875No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7090.1694085038768025No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7010.1674969833817187No Hit
GAAGATTCCGTCTTCACTTCCACCCGTGCGC6930.1655854628866349No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6820.16295712220589464No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6560.15674468059687227No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6340.1514879992353918No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6210.14838177843088063No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6100.14575343775014038No Hit
GGCGGCGGTGGCGCAAGCGGTGGCGGCGGTG6040.14431979737882752No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5920.1414525166362018No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA5690.13595689521283585No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5680.1357179551509504No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5670.13547901508906493No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5500.1314170340370118No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5440.12998339366569897No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5380.1285497532943861No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5330.12735505298495872No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5260.12568247255176038No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5180.12377095205667657No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5120.12233731168536373No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5110.12209837162347825No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5100.12185943156159279No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5090.12162049149970729No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5070.12114261137593635No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5070.12114261137593635No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4960.11851427069519611No Hit
TCTTCCGCTACTGCATGCACCGCAGGTGTTA4710.11254076914805922No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4680.11182394896240277No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4650.11110712877674635No Hit
GGCACTCTGGATCAGACCAACGGTCTGTTCG4630.11062924865297541No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4630.11062924865297541No Hit
GACGATGTGTGGACCAGCGGTTCTGACTCCG4540.10847878809600613No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA4460.10656726760092232No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4410.10537256729149494No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4380.1046557471058385No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4280.10226634648698374No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4210.10059376605378542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)