FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419924
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC55141.313094750478658No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG41260.9825587487259599No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38520.9173088463626751No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA33240.7915718082319658No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG29350.6989359979424848No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28070.6684542917289795No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG24800.5905830578866652No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC24170.5755803431097056No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA24070.5731989598117755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21900.5215229422466923No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC20280.48294453282022465No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20060.4777054895647784No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG19010.45270096493651235No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC17850.42507691868052316No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG15700.373877177775026No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14580.34720568483820885No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13300.3167239786247035No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC13230.31505701031615246No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC13190.3141044569969804No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13170.3136281803373944No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13100.3119612120288433No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13050.3107705203798783No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12710.3026738171669159No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11700.2786218458578219No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG11420.27195397262361765No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10230.2436155113782494No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9660.2300416265800478No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9340.22242120002667148No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8980.21384822015412314No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8800.20956173021784893No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8510.20265571865385165No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8350.19884550537716347No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8310.19789295205799146No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8300.19765481372819843No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7940.18908183385565006No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7890.187891142206685No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC7610.18122326897248076No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7490.17836560901496462No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6970.16598241586572807No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6890.164077309227384No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC6860.16336289423800496No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG6690.15931454263152378No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6400.15240853106752653No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6390.1521703927377335No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6340.15097970108876843No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6280.1495508711100104No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG6280.1495508711100104No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6280.1495508711100104No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT6070.14454996618435717No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC5940.14145416789704804No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5710.13597698631180882No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5360.12764214476905344No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5200.12383193149236528No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5140.12240310151360723No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5110.1216886865242282No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5090.12121240986464217No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC5060.12049799487526315No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4930.117402196587954No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT4820.11478267496023091No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC4810.11454453663043788No Hit
AACGGCGGCTGGTCCCACGACAACGATAATG4710.11216315333250777No Hit
ATCCACTCCCAGCGTGAACGTGACATGTTCC4670.11121060001333574No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4460.10620969508768255No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4360.10382831178975244No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4330.1031138968003734No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG4300.10239948181099438No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG4270.10168506682161534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)