FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538756
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28150.5224999814387219No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT26170.4857486505950746No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22760.4224546919199043No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC22620.41985611297136366No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19260.3574902182063866No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16980.3151705039015807No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG15190.2819458159166673No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG13860.25725931590553053No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13180.24463764672690422No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12930.23999732717593864No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG11290.2095568309216046No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10720.1989769023454031No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10020.18598400760269954No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9290.1724342745138801No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8910.16538098879641247No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA8440.15665718804059722No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7600.14106571434935297No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7060.13104262411926734No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6740.12510301509403143No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6630.1230612744916066No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG6450.11972024441491139No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6110.11340940982559823No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6100.11322379704355962No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6010.111553282005212No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG5970.11081083087705751No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC5820.10802663914647818No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5680.10542806019793746No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5640.10468560906978297No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5550.10301509403143538No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5540.10282948124939677No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5430.10078774064697191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)