FastQCFastQC Report
Wed 31 May 2023
EGAF00007931486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538756
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA59441.103282376437571No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49580.9202681733474894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48920.9080177297329403No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA30540.5668614363459525No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA29170.5414324852066612No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16630.3086740565302289No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG13400.24872112793175388No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12870.23888365048370688No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11500.21345469934441566No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10760.1997193534735576No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10360.1922948421920127No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9890.18357104143619746No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9660.17930194744930916No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9390.17429040233426635No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9260.17187743616776427No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG8740.1622255715017559No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8230.15275931961778616No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8090.15016074066924545No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8030.1490470639770137No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7090.1315994624653832No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7020.13030017299111285No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6950.12900088351684252No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6520.12101953388918174No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT6430.11934901885083415No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6250.11600798877413894No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6200.11507992486394583No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6180.1147086992998686No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5920.1098827669668644No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5820.10802663914647818No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5780.1072841880183237No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5630.10449999628774435No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5590.10375754515958988No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5480.10171580455716502No Hit
ATGGCTGAGTCCGACAAGCAGGCAGAACGCG5440.10097335342901052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)