FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497735
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56851.1421740484394305No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27660.55571739982119No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22070.443408641144384No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17080.34315448983897057No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15930.3200498257104684No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14880.29895426281053167No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14640.29413241986197475No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG14120.2836850934734346No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13490.2710277557334726No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13170.26459863180206333No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13030.26178589008207176No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12430.24973128271067938No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12180.24470852963926587No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG12020.24149396767356124No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11280.22662661858217728No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11040.22180477563362033No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG9500.1908646167137131No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9190.1846364029051604No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8400.1687645031994937No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8380.16836268295378062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8280.16635358172521522No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8250.1657508513566456No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8200.1647463007423629No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7960.15992445779380596No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7530.15128532251097473No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7100.14264618722814348No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7090.14244527710528695No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6960.13983344550815194No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6750.13561433292816458No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6440.12938611911961184No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6330.1271761077681899No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6330.1271761077681899No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6310.12677428752247683No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT6150.12355972555677219No Hit
ATTCCACAGGTTACCCGCAACGACACCGGCA5980.120144253468211No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5950.11954152309964139No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5490.11029965744824052No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5160.1036696233939747No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5120.10286598290254856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)