FastQCFastQC Report
Sun 21 May 2023
EGAF00007931556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482726
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55701.1538636825031177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43200.8949176137187556No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27560.5709242924557617No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21690.4493232185546252No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16260.3368370462746983No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15770.3266863603783513No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13840.2867050873580458No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13820.28629077364799077No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG13480.27924744057705614No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13400.27759018573683625No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13030.2699253821008191No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12890.26702518613043424No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11890.24630950062768525No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG11380.23574450102128328No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11100.22994410908051358No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10980.22745822682018368No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9680.20052783566661003No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9670.20032067881158255No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG9240.1914129340454005No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7960.16489685660188183No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7930.16427538603679934No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7910.16386107232674438No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7500.15536764127061728No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7240.14998156303990257No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6970.14438832795416034No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6970.14438832795416034No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6770.14024519085361054No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6470.13403048520278585No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6350.13154460294245596No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6190.12823009326201612No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6170.12781577955196116No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT6050.12532989729163127No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6000.12429411301649383No Hit
ATTCCACAGGTTACCCGCAACGACACCGGCA5650.11704362309053169No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5620.11642215252544923No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5560.11517921139528428No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5030.10419989807882732No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG4890.10129970210844247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)