FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487943
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA74361.5239484939839283No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57771.1839497646241468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44130.9044089166152603No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA39470.8089059582779137No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39450.8084960743365516No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA36200.7418899338652261No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG35760.732872487155262No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23860.48899154204487No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23550.48263834095375896No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23170.47485054606788085No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20060.4111135931860893No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC18500.37914264575985307No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16820.3447123946854448No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16560.3393839034477388No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15670.32114406805712964No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12080.24756990058265005No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11900.243880945110392No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11570.23711786007791893No Hit
AACCCGGAACACCTGAAGGAAGGCACTCAGA10770.2207225024234388No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10490.21498412724437077No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10300.21109022980143172No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9610.19694923382444263No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7760.1590349692484573No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7000.14345937947670118No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA6950.14243466962329615No Hit
GGTGTGCCGTCTGCCGGCCTGAAGGTTGGCG6850.14038524991648615No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6670.13669629444422812No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6600.1352617006494611No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6300.12911344152903106No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6200.12706402182222104No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6160.126244253939497No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6060.12419483423268701No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5890.12071082073110999No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5690.11661198131748995No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG5550.11374279372795591No Hit
GGCAAACAGGCGCTGGCTTTCGCACGTGAAC5510.11292302584523192No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5450.1116933740211459No Hit
GCGGGCAACGGTAGCTGCCGTTTCGACCAGA5380.1102587802263789No Hit
GCAGCTAACGTTGTACAGGTAGTTCAGAAAA5150.10554511490071586No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5050.10349569519390585No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4920.10083144957505283No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4880.10001168169232882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)