FastQCFastQC Report
Wed 31 May 2023
EGAF00007931602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487353
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT91881.8852864350891447No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33890.6953891737611136No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25660.5265177397081787No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25350.5201568472954922No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC17180.35251655370952883No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG16620.3410259093511274No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14830.30429688541980865No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14740.30245017471935126No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13930.28582977841523494No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12700.2605913988423176No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12620.2589498782196888No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11480.23555820934722882No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10910.2238623749109988No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9910.20334336712813914No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9870.20252260681682477No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9240.1895956319136232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9070.18610740059053704No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8080.16579358288550597No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7930.16271573171807704No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7560.15512369883841898No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7410.15204584767099003No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6650.1364514017560167No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6500.13337355058858774No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6440.13214241012161615No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6180.12680746809807267No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6130.12578151770892965No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5780.11859986498492878No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5660.11613758405098562No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5640.11572720389532844No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5440.1116234023387565No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5310.10895593132698475No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5260.10792998093784177No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5250.10772479086001317No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5230.10731441070435599No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5160.1058780801595558No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5120.10505731984824143No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5050.10362098930344124No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4940.10136389844732668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)