FastQCFastQC Report
Wed 31 May 2023
EGAF00007931603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473827
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA42200.8906204163122827No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA37860.7990258047768489No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24980.5271966350587872No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19420.40985422949726374No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG16690.35223826417658766No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15260.32205847281813826No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15040.31741542799376143No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13760.29040134901556897No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13100.27647221454243853No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11750.24798080312012613No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11310.23869471347137244No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10830.22856443385455028No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC10460.22075567664991652No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9360.19754045252803237No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9290.1960631200839125No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG9210.19437474014777545No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG8860.18698807792717595No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8190.17284789596202832No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8000.16883799361370289No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7160.1511100042842641No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6930.14625591196787013No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6780.1430901995876132No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6720.14182391463551042No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6680.1409797246674419No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6590.13908029723928775No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6460.13633667984306508No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG5580.11776450054555776No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5470.11544297813336934No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5460.11523193064135223No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5290.11164412327706104No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5240.1105888858169754No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5200.10974469584890689No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5090.10742317343671846No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5060.10679003096066707No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5050.10657898346864995No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG5010.10573479350058144No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC4930.10404641356444441No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4910.10362431858041016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)