FastQCFastQC Report
Sun 21 May 2023
EGAF00007931604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473827
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT90641.9129344676432538No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44940.948447429124976No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31900.6732414995346403No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25170.5312065374071127No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24390.5147448330297767No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16500.3482283618282622No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG15500.3271236126265494No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14370.30327524602861383No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14120.2979990587281856No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12840.27098497974999314No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12070.25473432286467423No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11890.2509354680083659No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11190.23616214356716692No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9620.20302768732047774No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9290.1960631200839125No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9130.19268636021163843No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8750.18466655551498753No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7610.16060714142503488No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7430.15680828656872656No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7150.15089895679224696No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6660.14055762968340765No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6420.13549248987499657No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6370.13443725241491092No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5770.1217744028938832No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5690.12008602295774619No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5510.11628716810143785No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5490.1158650731174036No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5300.11185517076907817No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5280.11143307578504391No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5270.11122202829302678No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5250.11079993330899252No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5210.109955743340924No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5050.10657898346864995No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5030.1061568884846157No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4960.1046795560404958No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT4950.10446850854847867No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC4950.10446850854847867No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4920.10383536607242727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)