FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482292
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG32220.6680600134358439No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA26930.5583754240169856No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC26540.5502890365172965No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT25110.5206389490184369No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24950.5173214567108723No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24330.504466174019059No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA19110.39623298748476027No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19040.39478158460020074No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18780.3893906596004081No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15490.3211747240261087No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14670.3041725759498395No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG13480.27949872691232697No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC11930.24736052018279384No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11660.2417622519137784No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10910.2262115067220688No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG10550.2187471490300482No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9940.20609920960745773No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9530.19759813556932312No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG9510.1971834490308776No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7680.15923963076310618No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7540.15633682499398704No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7530.15612948172476426No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7470.15488542210942746No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6700.1389199903792723No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6690.13871264711004952No Hit
ACGGCGGGCAACACGACGACGACGACGTTTCATTTTTGCGTAGC6500.13477312499481642No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6370.1320776624949201No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA6210.1287601701873554No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT6190.1283454836489098No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA6020.12482064807212227No Hit
GTAAGACAGCACCTGGCCCGGATAGATCAGGTTGGCGTTGGAGA5920.12274721537989434No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG5900.12233252884144875No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5900.12233252884144875No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5790.120051752879998No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5630.11673426057243329No Hit
AACGCTACGGATGTTCGCCACGCACAGGGTGTTGTTGGTAGACG5590.11590488749554212No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT5470.11341676826486857No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4940.10242757499606048No Hit
CGGATACAGTGCCGGGGCGTTATCACCGACCAGACCCAGGAAGC4860.10076882884227811No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4850.10056148557305532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)