FastQCFastQC Report
Sun 21 May 2023
EGAF00007931606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482292
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58381.2104700057226743No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA56051.1621590239937631No Hit
ATGTCCAACATGGACATCGATGGTATTAACA33140.6871355942043409No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27850.5774510047854827No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26540.5502890365172965No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25610.5310061124795767No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24730.5127599047879708No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA19270.39955047979232494No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16140.33465203652559034No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15710.32573627594901017No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12320.2554469076824828No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12220.25337347499025487No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11300.23429789422175779No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10330.21418559710714669No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10140.21024607499191358No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9750.20215968749222463No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9560.19822016537699153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8360.1733389730702562No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC8030.16649664518590396No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7940.1646305557628988No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7930.16442321249367603No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7860.16297180960911645No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7450.1544707355709819No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7200.14928715384041202No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6960.14431091537906496No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6960.14431091537906496No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6750.1399567067253863No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6680.13850530384082674No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6340.13145563268725172No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6330.1312482894180289No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6090.12627205095668184No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA6050.12544267787979066No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5920.12274721537989434No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA5840.121088469226112No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA5260.10906255961118991No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5250.1088552163419671No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5080.10533038076517959No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5050.10470835095751123No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5020.10408632114984283No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA4920.10201288845761489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)