FastQCFastQC Report
Sun 21 May 2023
EGAF00007931608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467290
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55201.1812792912324253No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA52681.1273513235892059No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43200.9244794453123327No Hit
ATGTCCAACATGGACATCGATGGTATTAACA30720.6574076055554366No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25730.5506216696269982No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24910.5330736801557919No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24900.5328596802841918No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24130.5163816901709859No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA17870.3824177705493377No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16090.34432579340452396No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15570.33319780008131994No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11040.23625585824648504No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC10920.23368785978728412No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10870.23261786042928376No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10280.2199918680048792No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9540.2041558775064735No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8930.1911018853388688No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG8240.1763358941984635No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7960.17034389779366133No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7650.16370990177405892No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7320.1566479060112564No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7310.1564339061396563No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7090.15172590896445462No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6940.14851591089045346No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6880.147231911660853No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6270.1341779194932483No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6270.1341779194932483No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6260.13396391962164822No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6220.13310792013524791No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6080.13011192193284685No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5970.127757923345246No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5790.12390592565644462No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA5620.1202679278392433No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5330.11406193156284106No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA5330.11406193156284106No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4850.10378993772603737No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA4840.10357593785443729No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4820.10314793811123714No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA4710.1007939395236363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)