FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483811
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG32160.6647223812604509No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28220.5832856218647364No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC26510.5479412415178655No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT26200.541533780753228No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24400.5043291698617849No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24140.4989551705107987No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA19630.40573695099946055No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC19050.3937487986011066No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18380.37990041565818056No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT16440.3398021128085141No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14400.2976368871315452No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12830.26518619874289756No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11340.23438904861609183No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10970.22674143415507297No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG10330.21351312806033762No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10250.21185958979849567No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG9940.20545212903385826No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9860.20379859077201634No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9820.2029718216410954No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8150.16845421042514536No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7750.16018651911593576No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7710.15935974998501481No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7110.14695821302120043No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6640.13724367573287916No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6600.1364169066019582No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA6450.1333165223610046No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6380.13186967638189295No Hit
ACGGCGGGCAACACGACGACGACGACGTTTCATTTTTGCGTAGC6280.12980275355459053No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG6270.1295960612718603No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5970.1233952927899531No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5960.12318860050722286No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5940.12277521594176238No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5790.11967483170080878No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5760.11905475485261807No Hit
GTAAGACAGCACCTGGCCCGGATAGATCAGGTTGGCGTTGGAGA5490.11347406321890159No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT5370.11099375582613873No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5050.10437960277877105No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG4940.10210598766873841No Hit
AACGCTACGGATGTTCGCCACGCACAGGGTGTTGTTGGTAGACG4920.10169260310327792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)