FastQCFastQC Report
Wed 31 May 2023
EGAF00007931610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483811
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61081.262476462916304No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA55041.1376343241472393No Hit
ATGTCCAACATGGACATCGATGGTATTAACA32620.6742302262660419No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA26130.5400869347741163No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26030.5380200119468139No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25940.5361597814022419No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25890.5351263199885906No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA19550.4040834127376186No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17200.35551072629601227No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15930.32926080638927185No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12490.2581586611300694No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC11270.23294220263698015No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10860.22446781904504032No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10810.22343435763138914No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10300.21289305121214688No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG10170.21020605153665378No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9890.20441866762020708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8880.18354274706445284No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8380.17320813292794088No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8040.16618059531511273No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC7840.16204674966050794No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7640.15791290400590313No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA7310.15109205867580522No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7300.15088536639307498No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7290.15067867411034475No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6960.14385782878024683No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6720.13889721399472107No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6630.13703698345014892No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6610.13662359888468845No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6370.1316629840991627No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6370.1316629840991627No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6190.12794252301001838No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA5610.11595437061166446No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5600.11574767832893423No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA5410.11182052495705967No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5210.10768667930245489No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5160.10665321788880368No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5100.10541306419242225No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA4840.100039064841436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)