FastQCFastQC Report
Wed 31 May 2023
EGAF00007931636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468621
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48511.0351648773742532No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG39600.8450325529585742No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31270.6672769679549145No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT30370.6480716826604015No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA28780.6141423453067617No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26960.5753049906000798No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19630.4188886114792124No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18430.3932815644198616No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17880.38154500118432594No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16380.34953619236013755No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG14600.31155240588876726No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC14060.30002923471205944No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT14040.29960245059440355No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13790.2942676491237055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13190.28146412559403017No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12750.2720748750056015No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11490.24518747559328324No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10080.21509919529854615No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9900.21125813823964354No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9620.2052831605924617No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9180.19589391000403314No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9160.19546712588637727No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8920.19034571647450713No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8440.1801028976507668No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8370.17860915323897136No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7990.1705002550035103No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7570.16153778853273756No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6840.14596016823829916No Hit
GGTATTGTTTCCAACAACTCTATTCGCATCG6210.13251646853214005No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6200.13230307647331213No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6040.12888880353206536No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5810.12398078617902313No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5600.11949955294363675No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5410.11544510382590623No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4930.10520228500216593No Hit
GTGAGCGATGCCGTCGCTAACGGCCTGAAGG4770.10178801206091916No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4770.10178801206091916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)