FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456580
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC46701.0228218494020762No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39250.8596521967672697No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37250.815848263174033No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT32120.703491173507381No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29000.6351570371019317No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG27850.6099697752858206No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27100.5935433001883569No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18720.41000481843269526No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18620.40781462175303346No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17340.37978010425336195No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT14780.32371106925401905No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14560.318892636558763No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG14540.3184545972228306No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13410.2937053747426519No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12510.2739936046256954No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11590.2538437951728065No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11330.24814928380568574No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9560.2093828025756713No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9270.203031232204652No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9120.1997459371851592No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9090.1990888781812607No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9030.19777476017346357No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8690.19032809146261334No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8100.17740593105260852No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7620.16689298699023172No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7180.15725612159971966No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7170.15703710193175346No Hit
GGTATTGTTTCCAACAACTCTATTCGCATCG6700.14674317753734287No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6430.1408296465022559No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5650.1237461124008936No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5440.11914669937360374No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5330.11673748302597572No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5220.11432826667834772No Hit
GTGAGCGATGCCGTCGCTAACGGCCTGAAGG5170.1132331683385168No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5140.11257610933461824No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4930.1079766963073284No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4790.10491042095580182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)