FastQCFastQC Report
Wed 31 May 2023
EGAF00007931784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514239
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76321.4841348089118096No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA34390.6687551897075096No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT28440.5530502353963818No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15420.2998605706685024No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13010.2529952026197935No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11690.2273262043524509No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10280.19990704711233495No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10240.19912919867999124No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG8790.1709321930075315No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8080.15712538333343057No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8050.1565419970091728No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7990.1553752243606572No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7270.14137395257847032No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7240.14079056625421255No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6590.12815052922862716No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6460.12562252182351008No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5360.10423168993405789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)