FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007931830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454831
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66521.4625212441544222No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA56261.236942952437279No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27760.6103365865563254No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22730.4997460595254062No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22350.4913913079803268No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21990.48347628020077793No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19550.4298299808060576No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16090.3537577693692822No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14420.317040834947486No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14350.31550180176812925No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14140.3108847022300591No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12620.27746569604974153No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12480.2743876296910281No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC12240.26911094450466216No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10670.2345926289105184No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10620.2334933194966922No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC9930.21832284958589016No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9090.19985445143360941No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9070.19941472766807894No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8630.18974080482640804No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8520.18732232411599034No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7980.175449782446667No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7810.1717121304396578No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7660.1684142021981791No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7540.16577585960499613No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7390.16247793136351743No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7340.16137862194969121No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT6990.15368345605290756No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6480.14247050003187997No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5940.13059795836255664No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5870.12905892518319992No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5840.12839933953490418No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5790.12730003012107793No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5510.12114389740365103No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5480.12048431175535528No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5410.11894527857599857No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5100.1121295602102759No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5050.11103025079644967No Hit
GAATGCGACAACTATCGCCAGGAATACGATA4700.10333508489966603No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4630.10179605172030931No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4620.10157618983754405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)