FastQCFastQC Report
Wed 31 May 2023
EGAF00007931894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527192
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT83821.589933079409399No Hit
ATGTCCAACATGGACATCGATGGTATTAACA34570.6557383268334877No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA28370.5381341143264693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24980.4738311658750512No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18420.349398321674077No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG15690.2976145313282447No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG15180.2879406364284739No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15140.2871818995735899No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13570.25740147801939334No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG13210.2505728463254374No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13140.24924505682939044No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12990.24639979362357545No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12970.24602042519613349No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12570.23843305664729358No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11720.22230989848100882No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11340.21510189835961094No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG11330.2149122141458899No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11160.21168758251263295No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10530.19973747704821015No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10510.19935810862076817No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC10490.19897874019332618No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10060.19082231900332328No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9690.18380400309564637No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9150.17356105555471252No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8900.16881895021168758No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8740.16578400279215164No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8600.16312842380005768No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8420.15971410795307972No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8220.15592042367865977No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7610.14434968664167894No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC7270.13790042337516503No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7110.13486547595562906No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7080.1342964233144661No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7020.1331583180321401No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7000.1327789496046981No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6970.1322098969635351No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6840.12974400218516213No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6660.12632968633818417No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6280.11912168621678629No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6260.1187423177893443No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6220.1179835809344603No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5990.11362084401887737No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5880.1115343176679464No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5830.11058589659934141No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5800.11001684395817843No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5640.10698189653864246No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5440.10318821226422252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)