FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493771
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA44940.9101385055015381No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT30520.6181002934558733No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23870.483422477221222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23710.4801821087103131No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT23470.4753215559439497No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT21100.42732359737611164No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19150.3878316061494093No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18400.3726423787545239No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18290.37041462540327397No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG18240.36940201024361496No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17610.35664305923191114No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG16360.33132768024043535No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15710.3181636831648679No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC14390.2914306429498695No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA14340.2904180277902104No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG14190.28738018231123336No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13550.27441870826759773No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13260.26854554034157535No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA13130.26591274092646183No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12980.26287489544748477No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12930.26186228028782577No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12900.2612547111920303No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC12430.2517361286912354No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12290.24890080624419011No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11860.24019231587112244No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11820.2393822237433952No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10940.22156019693339626No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10560.21386432171998762No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG10110.20475078528305632No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC9950.2015104167721474No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9870.19989023251669297No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9200.18632118937726194No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8910.18044802145123956No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8360.16930925469499017No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8170.16546131708828588No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7980.16161337948158155No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7910.1601957182580589No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7580.15351245820430928No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7550.15290488910851388No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA7490.15168975091692302No Hit
ATCGAAACCGTCGGTGACATCGTGTCCGGTC7310.1480443363421505No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7190.14561405995896884No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7020.14217116841612812No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6980.14136107628840092No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6690.13548790836237853No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6120.12394409554226553No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6030.12212138825487928No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5930.12009615793556122No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5760.11665326639272051No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5720.11584317426499328No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5600.11341289788181161No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5590.11321037484987981No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5550.11240028272215256No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5410.10956496027510727No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5390.10915991421124369No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5320.10774225298772103No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5200.10531197660453935No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5020.10166656202976684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)