FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549131
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68181.2415980886163775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47120.8580830439366928No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27760.5055260038132977No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19380.35292125194170426No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT19340.3521928283050857No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG16540.30120317374178474No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16090.2930084078298257No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16040.2920978782840524No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15970.2908231369199699No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15760.28699891282772233No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG14870.27079148691295885No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13310.24238296508483403No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA13270.24165454144821547No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12930.23546294053695746No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC12460.22690396280668912No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12200.22216920916866834No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12110.22053025598627649No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11780.2145207609841732No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11560.21051443098277098No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11230.20450493598066763No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10890.19831333506940968No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10510.1913933105215331No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10300.18756908642928555No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9960.18137748551802757No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9400.1711795546053674No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9300.16935849551382093No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9200.16753743642227448No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA8650.15752161141876891No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7820.1424068209589333No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7450.1356689023202114No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7120.12965940731810807No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT7030.12802045413571625No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6770.12328570049769545No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6630.1207362177695304No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6510.11855094685967464No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6490.11818673504136537No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6290.11454461685827244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)