FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559455
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT74261.3273632374364337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46730.8352771894075484No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28460.5087093689394142No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT20570.3676792592791198No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT19150.34229741444798956No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16970.30333092027062053No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG16800.30029224870633026No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16130.288316307835304No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15860.28349018240966656No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15670.28009402007310685No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG15160.27097800538023614No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14900.2663306253407334No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14200.25381844831130296No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA13090.23397771045034899No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG12740.22772162193563378No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12410.22182302419318803No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC12290.21967807955957136No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12250.21896309801503247No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11710.20931084716375759No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11470.20502095789652428No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11430.20430597635198539No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10850.19393874395617164No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10470.18714641928305226No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10290.1839290023326273No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10240.1830352754019537No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9960.17803040459018152No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9000.16087084752124836No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA8530.15246981437291648No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT8010.14317505429391103No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7770.13888516502667775No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6990.12494302490816955No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6890.12315557104682236No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC6890.12315557104682236No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6640.11868693639345435No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6430.11493328328462521No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6400.11439704712622105No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6250.11171586633420025No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6070.10849844938377529No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6000.10724723168083222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)