FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429047
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44661.040911601759248No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA43771.020167953627458No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23260.5421317478038536No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22670.5283803406153639No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19680.4586910058804746No Hit
ATGTGCGAAGACACCATCACCTACGAATGTC11820.27549429316601676No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11530.26873512692082685No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG11050.257547541411547No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10450.24356305952494714No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9500.22142096320449742No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9390.21885714152528746No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9340.21769176803473744No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9050.21093260178954754No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8800.20510573433679763No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8780.2046395849405776No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8280.19298585003507776No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8140.1897228042615378No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7570.17643754646926793No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7320.170610679016518No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6980.1626861392807781No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6790.15825772001668814No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6760.15755849592235815No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6100.14217556584709834No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5860.1365817730924584No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5760.13425102611135842No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5530.12889030805482848No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5310.12376266469640855No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5150.1200334695266486No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5050.11770272254554863No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA4880.11374045267767867No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4710.10977818280980872No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4570.10651513703626876No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4510.10511668884760877No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG4450.10371824065894877No Hit
GTAGGTCCAACCCCTCACCAGACCCCGCAGG4360.10162056837595881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)