FastQCFastQC Report
Wed 31 May 2023
EGAF00007931974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409995
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47211.151477457042159No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17250.42073683825412506No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14190.3461017817290455No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13420.32732106489103524No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT13020.31756484835180915No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11320.2761009280600983No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG9500.2317101428066196No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8850.21585629093037723No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8590.20951475017988025No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG8190.19975853364065416No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7950.1939048037171185No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG7820.19073403334187003No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7280.1775631410139148No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7030.1714655056768985No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6640.16195319455115306No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG6450.15731899169502067No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5750.14024561275137504No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5540.13512359906828134No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5140.12536738252905524No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5020.12244051756728741No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4900.11951365260551958No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4850.11829412553811632No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT4820.11756240929767436No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4790.11683069305723241No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4780.11658678764375176No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4640.11317211185502263No Hit
GAAACTCTGTATCAGAAAACCGACGCAGGTA4460.10878181441237088No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4310.1051232332101611No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4260.10390370614275783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)