FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007931994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475495
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT66141.3909715138960452No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG27080.5695117719429227No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT26030.5474295208151505No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG25020.5261884983017697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24420.5135700690859No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19980.4201936928884636No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17400.36593444726022356No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA15480.3255554737694403No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12620.265407627840461No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12190.25636442023575434No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9350.1966371886139707No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9240.19432380992439457No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9210.19369288846360108No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG9140.19222073838841627No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8980.188855823930851No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8870.1865424452412749No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8850.18612183093407925No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8720.18338783793730742No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8720.18338783793730742No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG8420.17707862332937255No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7980.16782510857106805No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7720.16235712257752447No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7660.1610952796559375No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7640.16067466534874184No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7610.16004374388794834No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7470.15709944373757873No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7010.14742531467207856No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6850.14406040021451327No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6460.13585842122419794No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6350.1335450425346218No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG5690.11966477039716507No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG5530.11629985593959978No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5530.11629985593959978No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5480.11524832017161064No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5440.11440709155721931No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5410.11377617009642584No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5370.11293494148203452No Hit
ATGGCATCCACCACCCCGATCACCATGGAAG5250.11041125563886055No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5010.10536388395251264No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA4760.1001062051125669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)