FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007931996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007931996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458303
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61851.349543860720964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48281.0534515375199376No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG26540.579092870873636No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24290.5299987126420731No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG23890.5212708622897952No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18790.4099907702982524No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15850.3458410702090102No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA13970.30482017355330426No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12460.2718725384734553No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11440.24961652007514679No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9000.19637663292625182No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9000.19637663292625182No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG8880.1937582778205685No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8840.1928854927853407No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8720.19026713767965733No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8530.18612140876232536No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8410.18350305365664202No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8050.17564798833959194No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7930.17302963323390858No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7810.1704112781282252No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7790.1699748856106113No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7690.16779292302254184No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7500.16364719410520986No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7450.16255621281117513No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7430.16211982029356126No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7400.1614652315171404No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6560.1431367457773569No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6560.1431367457773569No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6540.142700353259743No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5850.1276448114020637No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5390.11760778349694416No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG5350.11673499846171637No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5350.11673499846171637No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC5300.11564401716768163No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4990.10887993314466629No Hit
ATGGCATCCACCACCCCGATCACCATGGAAG4960.10822534436824545No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG4900.10691616681540378No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4750.10364322293329958No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4690.10233404538045791No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA4650.10146126034523012No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA4650.10146126034523012No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4640.10124306408642318No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4620.10080667156880928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)