FastQCFastQC Report
Sun 21 May 2023
EGAF00007932136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452729
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62971.390898307817701No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25110.5546364381340714No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23090.5100181344689649No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16380.3618058485319032No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15120.33397462941406447No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10550.23303123943904633No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10350.22861358561081796No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9620.2124891491377844No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9340.20630443377826468No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9300.205420903012619No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9070.20034060111015642No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8930.19724824343039654No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8650.19106352807087684No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8540.18863381846535124No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8500.18775028769970556No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8490.18752940500829415No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7820.17273026468372912No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7320.1616861301131582No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7210.1592564205076326No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6860.15152552630823296No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6800.15020023015976444No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6380.14092315712048487No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6110.1349593244523766No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6100.13473844176096517No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5900.1303207879327368No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5840.1289954917842683No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5840.1289954917842683No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5830.12877460909285687No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5560.12281077642474858No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5550.12258989373333715No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5420.11971841874498872No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5330.11773047452228597No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5270.11640517837381746No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5100.11265017261982332No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5000.11044134570570914No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4860.1073489880259493No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4740.10469839572901228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)