FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007932138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456319
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64161.4060339367854506No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25570.560353612275623No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22750.49855473911890585No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16790.3679443547167661No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15420.3379214978994957No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10390.22769159294265634No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10240.22440441883857565No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10230.22418527389830362No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9970.21848750545123036No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9750.21366631676524536No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9720.21300888194442924No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9270.20314735963218714No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9020.19766873612538596No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9010.1974495911851139No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8920.1954772867226655No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8670.18999866321586434No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8310.18210944536607068No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7470.16370127038321877No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7090.155373762652881No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7090.155373762652881No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6800.14901855938499164No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6380.1398144718935657No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6320.13849960225193342No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6110.13389755850622043No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6110.13389755850622043No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5930.12995294958132358No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5830.12776150017860313No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5830.12776150017860313No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5800.127104065357787No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5650.12381689125370629No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5630.12337860137316219No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5470.11987228232880945No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5360.11746168798581695No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5010.10979161507629531No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4980.10913418025547918No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4860.10650444097221462No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4700.10299812192786185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)