FastQCFastQC Report
Wed 31 May 2023
EGAF00007932174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503379
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA59481.1816146482074141No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37520.7453628379411934No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA31690.6295455312994781No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA29910.5941845011412872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29870.5933898712500918No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22810.453137695454121No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22120.4394303298310021No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17790.35341164410911063No Hit
GAACGTACCACTGACGGTAAACTGGATCCGC15620.3103029725117655No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13540.2689822181696098No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11600.23044266844663763No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11350.225476231626667No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11110.22070845227949518No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9670.1921017761964643No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9040.1795863554101383No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC9030.17938769793733947No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8340.1656803323142205No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8180.16250181274943928No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7300.14501995514314264No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6880.13667634128559197No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6860.1362790263399943No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6350.1261474952272542No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6010.11939314115209415No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5980.11879716873369767No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5930.11780388136970354No Hit
GGCTGCGACCGTGCACCGTCTTCCGGTGAAA5520.1096589249849517No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5490.10906295256655522No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5200.10330188585538928No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5100.10131531112740104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)