FastQCFastQC Report
Wed 31 May 2023
EGAF00007932194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502948
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57051.1343120958826758No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA30500.6064245210240422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29380.5841558173011921No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA29200.5805769184885913No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC22740.45213421665858106No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC21890.4352338611546323No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC17150.3409895257561418No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC17030.33860359321440786No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15250.3032122605120211No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15120.300627500258476No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14600.29028845924429564No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14570.28969197610886216No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC14450.28730604356712824No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14390.28611307729626123No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13090.26026547476081024No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA12970.25787954221907633No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10470.20817261426628597No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG10260.2039972323182516No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10210.2030030937591958No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG9190.1827226671544573No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8850.17596252495287784No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8520.1694012104631095No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8250.16403286224420816No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8230.16363520682058583No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG7750.15409147665365008No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7660.15230202724734962No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7550.15011492241742685No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC7300.14514422962214782No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7020.1395770536914353No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7020.1395770536914353No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6570.13062980665993304No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6470.12864152954182143No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6190.1230743536111089No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5840.11611538369771825No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5780.11492241742685129No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5730.11392827886779547No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5640.11213882946149503No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5460.10855993064889412No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5400.10736696437802715No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5230.10398689327723741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)