FastQCFastQC Report
Sun 21 May 2023
EGAF00007932196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007932196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506546
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT61811.2202248166997667No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA29870.5896799106102901No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29630.5849419401199496No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA29350.5794143078812191No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC22570.445566641529101No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC21500.42444318975966644No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC16930.33422433500610016No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16450.3247483940254192No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG15840.3127060523624705No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15800.31191639061408044No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15150.2990843872027417No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14830.2927670932156211No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14550.2872394609768905No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14010.2765790273736245No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13530.26710308639294356No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA13290.26236511590260314No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10890.2149854109991985No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10260.2025482384620548No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG10220.20175857671366468No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9310.18379377193779045No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8970.17708164707647478No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG8760.17293592289742687No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8690.17155401483774424No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8430.16642121347320873No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7490.14786416238604194No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7420.1464822543263593No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC7290.14391585364409157No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG7210.1423365301473114No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7150.14115203752472627No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7110.1403623757763362No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6460.12753037236499745No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6450.12733295692789992No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6010.11864667769560908No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5970.11785701594721901No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5830.11509319982785374No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5530.10917073671492816No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5490.1083810749665381No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5420.10699916690685544No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5170.10206378097941747No Hit
GCAGCTCGTGAAGAAGAAGAACCGCACGAAC5070.10008962660844227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)